Bilirubin Oxidase antibody (HRP)

Catalog No. ABIN5596696

Product Details anti-Bilirubin Oxidase Antibody (HRP)

Target: Bilirubin Oxidase

Reactivity: Myrothecium verrucaria

Host: Goat

Clonality: Polyclonal

Conjugate: This Bilirubin Oxidase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Bilirubin Oxidase from other sources is unknown.

Purification: Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].

Immunogen:

Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Bilirubin Oxidase has been assayed against 1.0 μg of Bilirubin Oxidase [Myrothecium verrucaria] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Comment:

User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Bilirubin Oxidase

Target: Bilirubin Oxidase

Abstract: Bilirubin Oxidase Products

Synonyms: Bilirubin oxidase antibody, bilirubin oxidase antibody, hypothetical protein antibody, Franean1_4816 antibody, Dole_0431 antibody, SARE_RS06935 antibody, PTRG_09905 antibody, Chy400_1070 antibody, Bcav_4005 antibody, Caci_0156 antibody, Hneap_0275 antibody, Sthe_0428 antibody, HTUR_RS21015 antibody, TK90_1924 antibody, BC1002_7184 antibody, Gobs_1113 antibody, Toce_1552 antibody, AciX9_0181 antibody, NAL212_2028 antibody, Psed_5122 antibody, Lbuc_2174 antibody, Halhy_6631 antibody, HALXA_RS20230 antibody

Background:

Synonyms: Bilirubin Oxidase

Background: Bilirubin Oxidase is key in the oxidation of bilirubin and other tetrapyrroles. This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-CH group of donor with oxygen as acceptor. The systematic name of this enzyme class is bilirubin:oxygen oxidoreductase. This enzyme is also called bilirubin oxidase M-1. This enzyme participates in porphyrin and chlorophyll metabolism.

Gene Name: BLRO_MYRVE

UniProt: Q12737