V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (EQKLISEEDL) antibody

Antigen: V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyms: MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746

Epitope: EQKLISEEDL

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116942

Additional Information

Characteristics: Synonyms: myc tag, myc-tag, c-myc tag

Alternative name: C-Myc Epitope Tag (EQKLISEEDL)

Gene ID: 4609

Swiss-Prot: P01106

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizationswith Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. Remarks: The sequence corresponds to aa 410-419 of Human c-Myc.

Description: Epitope tags are short peptide sequences that are easily recognized by tag-specificantibodies. Due to their small size, epitope tags do not affect the tagged protein'sbiochemical properties. Most often sequences encoding the epitope tag are included withtarget DNA at the time of cloning to produce fusion proteins containing the epitope tagsequence. This allows anti-epitope tag antibodies to serve as universal detection reagentsfor any tag containing protein produced by recombinant means. This means thatanti-epitope tag antibodies are a useful alternative to generating specific antibodies toidentify, immunoprecipitate or immunoaffinity purify a recombinant protein. Theanti-epitope tag antibody is usually functional in a variety of antibody-dependentexperimental procedures. Expression vectors producing epitope tag fusion proteins areavailable for a variety of host expression systems including bacteria, yeast, insect andmammalian cells. We offer anti-epitope tag antibodies against many common epitope tagsincluding Myc, GST, GFP, 6X His, MBP, FLAG and HA. Please visit our website atwww. acris-antibodies. de for more informations.

Specificity: Anti-Myc has utility to detect the fusion protein of the myc epitope cloned along with thetarget gene. As such, anti-myc/myc can be used to identify fusion proteins containing themyc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxyterminiof targeted proteins. This affinity purified antibody is directed against human c-myc and is useful in determiningits presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Mycepitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu)fused to either the amino- or carboxy- termini of targeted proteins in transfected ortransformed cells.

Application Details

Application Notes: This antibody has been tested by ELISA (1/135,000) and Western blot (1/500-1/5,000)against both the immunizing peptide and myc containing recombinant proteins. Use1/400-1/2,000 for Immunohistochemistry on frozen sections. Although not tested, this antibody is likely functional for Immunoprecipitation andImmunocytochemistry.

Concentration: 1.17 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservative without stabilizers.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Transcription Factors, Tags/Labels

Restrictions: For Research Use only

Images

Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by Western blot. Polyclonal rabbit-anti-Myc epitope tag antibody was diluted to 1.0 ug/ml to detect either recombinant protein. 4-20% gradient gels were used to separate the proteins by SDS-PAGE. The proteins were transferred to nitrocellulose using standard methods. After blocking the membranes were probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (Green 800 nm channel). Pre-stained molecular weight markers are also shown (lane M, Red 700 nm channel). LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.