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Antibodies
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anti-Avidin antibody (TR (Texas Red))
Avidin antibody (TR (Texas Red))
| Antigen |
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| Synonyms |
LOC396260, AVD, avidin |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
Western Blotting (WB) (15), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), ELISA (6), Immunoprecipitation (IP) (4), Immunohistochemistry (IHC) (3), ELISA (Capture) (2), Flow Cytometry (FACS) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Dot Blot (Dot) (1), ELISA (Detection) (1), Immunofluorescence (IF) (1)
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| Catalog no. |
ABIN116965 |
| Quantity |
20 mg (10.0 mg/ml (by UV absorbance at 280...) |
| Price |
350.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: AVD
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Swiss-Prot
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P02701
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Immunogen
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Avidin [Hen Egg White].
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Description
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The biological function of avidin is not known. It is a homotetrameric glycoprotein found inchicken egg white, and forms a strong non covalent specific complex with biotin (onemolecule of biotin per subunit of avidin).
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Specificity
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This product was prepared from monospecific antiserum by delipidation, salt fractionationand ion exchange chromatography followed by extensive dialysis against the buffer statedabove. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-RabbitSerum and anti-Avidin. No reaction was observed against Streptavidin.
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Application Notes
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Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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10.0 mg/ml (by UV absorbance at 280 nm)
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Buffer
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0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/mlBovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodiumazide as preservative.Label: Texas Red(TM) Sulfonyl Chloride (Molecular Weight 625 daltons). Absorp
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Storage
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Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and thenaliquot contents and freeze at -20°C or below. Centrifuge product if not completely clearafter standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Epitope Tags, Tags/Labels
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Restrictions
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For Research Use only
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Alternatives for antigen "Avidin", type "Antibodies"
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Hosts
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Rabbit (17), Mouse (4)
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Reactivities
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Chicken (8), Human (1)
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Applications
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Western Blotting (WB) (15), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), ELISA (6), Immunoprecipitation (IP) (4), Immunohistochemistry (IHC) (3), ELISA (Capture) (2), Flow Cytometry (FACS) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Dot Blot (Dot) (1), ELISA (Detection) (1), Immunofluorescence (IF) (1)
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Conjugates
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Biotin (3), FITC (3), HRP (3), Cy5.5 (1), Texas Red (TR) (1)
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