CD22 antibody (pTyr822) (PE)

Catalog No. ABIN1177229

Product Details anti-CD22 Antibody (PE)

Target: CD22 (CD22 Molecule (CD22))

Binding Specificity: pTyr822

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is conjugated to PE

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Mouse CD22 Peptide

Clone: 12a-CD22

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD22 antibody (PE) (ABIN1177229)

Tedder, Tuscano, Sato, Kehrl: "CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling." in: Annual review of immunology, Vol. 15, pp. 481-504, (1997) (PubMed).

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22 (CD22 Products)

Background: CD22 is a member of the immunoglobulin (Ig) superfamily that contains several extracellular Ig domains and cytoplasmic Immunoreceptor Tyrosine-based Inhibitory Motifs (ITIMs). It is expressed in the cytoplasm of pro-B and pre-B cells and on the surface of IgD-positive mature B lymphocytes. Differential splicing of the human CD22 gene results in the expression of two isoforms, the full-length CD22b has 7 Ig domains, while CD22a has only 5. CD22b is the predominant isoform in human cells, at both the mRNA and protein levels. Ligands for the extracellular domains of CD22 are sialic acid-linked glycoproteins on the B cell surface. The intracellular ITIM motifs are substrates of src family tyrosine kinases. Signal transduction is initiated by the phosphorylation of tyrosines in the ITIMs of CD22 upon ligation of the B cell receptor (BCR). Tyrosine phosphorylation in ITIM3 of human CD22 or ITIM2 of mouse CD22 is required for recruitment of the protein tyrosine phosphatase PTP1C (SHP-1), which may terminate signal transduction by CD22 and by co-localized receptors, such as the BCR. Studies of mice deficient for CD22 suggest that this adhesion molecule generates both negative and positive regulatory signals that affect B cell development and responsiveness. The 12a/CD22 monoclonal antibody recognizes the phosphorylated tyrosine 822 (pY822) in ITIM3 of human CD22b. The equivalent site in human CD22a is pY645. The orthologous phosphorylation site in the predominant isoform of mouse CD22 is pY837, which is in ITIM2. We recommend the PE conjugate of mAb 12a/CD22, Cat. No. 560076, for staining both human and mouse leukocytes.
Synonyms: Human BL-CAM, Siglec-2, or Leu-14 (pY822), Mouse Lyb-8 (pY837)