CD22 antibody (CD22 Molecule) (PE)

Details for Product anti-CD22 Antibody No. ABIN94037
Antigen
Reactivity
Human, Non-Human Primate
422
149
44
15
4
3
3
3
2
2
Host
Mouse
297
152
72
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD22 antibody is conjugated to PE
64
50
34
25
7
7
6
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
333
179
105
103
95
44
34
22
14
13
12
11
4
4
3
3
2
2
2
1
Options
Immunogen Raji Burkitt's lymphoma cell line
Clone MEM-01
Isotype IgG1
Specificity The antibody MEM-01 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes, it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E", it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
Cross-Reactivity (Details) Human, Non-Human Primates
Antigen
Alternative Name CD22 (CD22 Antibody Abstract)
Background CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD22 Molecule (CD22) antibody (PE) (ABIN94037) Surface staining of human peripheral blood cells with anti-CD22 (MEM-01) PE. Cells in...