CD22 antibody (CD22 Molecule)

Details for Product anti-CD22 Antibody No. ABIN94034
Antigen
Reactivity
Human, Non-Human Primate
422
149
44
15
4
3
3
3
2
2
Host
Mouse
297
152
72
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD22 antibody is un-conjugated
65
50
34
25
7
7
6
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
333
179
105
103
95
44
34
22
14
13
12
11
4
4
3
3
2
2
2
1
Options
Immunogen Raji Burkitt's lymphoma cell line
Clone MEM-01
Isotype IgG1
Specificity The antibody MEM-01 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes, it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E", it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
Cross-Reactivity (Details) Human, Non-Human Primates
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD22 (CD22 Antibody Abstract)
Background CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD22 Molecule (CD22) antibody (ABIN94034) Surface staining of human peripheral blood cells with anti-CD22 (MEM-01) PE. Cells in...