ZNF148 antibody

Catalog No. ABIN117932

The Rabbit Polyclonal anti-ZNF148 antibody has been validated for WB and EIA. It is suitable to detect ZNF148 in samples from Human.

Quick Overview for ZNF148 antibody (ABIN117932)

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF148 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ZNF148 Antibody

Sequence: 1 mniddklegl flkcggidem qssrtmvvmg gvsgqstvsg elqdsvlqdr smphqeilaa 61 devlqesemr qqdmishdel mvheetvknd eeqmetherl pqglqyalnv pisvkqeitf 121 tdvseqlmrd kkqirepvdl qkkkkrkqrs pakiltined gslglktpks hvcehcnaaf 181 rtnyhlqrhv fihtgekpfq csqcdmrfiq kyllqrheki htgekpfrcd ecgmrfiqky 241 hmerhkrths gekpyqceyc lqyfsrtdrv lkhkrmchen hdkklntcai kgglltseed 301 sgfstspkdn slpkkkrqkt ekkssgmdke saldksdlkk dkndylpvys sstkvkdeym 361 vaeyavemph ssvggshled asgeihppkl vlkkinskrs lkqpleqnqt isplstyees 421 kvskyafelv dkqalldseg nadidqvdnl qegpskpvhs stnyddamqf lkkkrylqaa 481 snnsreyaln vgtirsqpsv tqaavasvid esttasiles qalnveiksn hdknvipdev 541 lqtlldhysh kangqheisf svadtevtss isinssevpe vtpsenvgss sqasssdkan 601 mlqeyskflq qaldrtsqnd aylnspslnf vtdnqtlpnq pafssidkqv yatmpinsfr 661 sgmnsplrtt pdkshfgliv gdsqhsfpfs gdetnhasat stqdfldqvt sqkkaeaqpv 721 hqayqmssfe qpfrapyhgs ragiatqfst angqvnlrgp gtsaefsefp lvnvndnrag 781 mtsspdattg qtfg

Specificity: This polyclonal antibody is specific for ZBP-89.

Purification: Delipidation and defibrination

Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli

Application Details

Application Notes: This polyclonal antibody reacts human ZBP-89 in a variety of immunological assaysincluding western blot and ELISA. This antibody is likely functional inimmunohistochemistry and immunoprecipitation. Recommended dilutionsImmunoblotting: 1: 5,000. A band at approximately 89 kDa corresponding to human ZBP-89is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937cells can be used as a positive control. ELISA: 1: 10,000 - 1: 30,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 90 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Alternative Name: ZNF148

Background: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The fulllength protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.Synonyms: Transcription factor ZBP-89, ZBP89, Zinc finger DNA-binding protein 89, Zinc finger protein 148

Gene ID: 7707

NCBI Accession: NP_068799

UniProt: Q9UQR1