SET Domain, Bifurcated 1 (SETDB1) antibody

Antigen: SET Domain, Bifurcated 1 (SETDB1)

Synonyms: ESET, KG1T, KMT1E, KIAA0067, H3-K9-HMTase4, AU022152, MGC90670, mKIAA0067, SETDB1, NV10069, fc07d03, wu:fc07d03, si:ch211-81a5.6

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN117938

Additional Information

Alternative name: SETDB1

UniProt: Q15047

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic peptide corresponding to amino acids 1058-1075 of humanESET. AA Sequence: Human ESET, 1291 aa, predicted MW 143. 2 kDa: 1 msslpgcigl daatatvese eiaelqqavv eelgismeel rhfideelek mdcvqqrkkq61 laeletwviq kesevahvdq fddasravt nceslvkdfy sklglqyrds ssedessrpt121 eiieipdedd dvlsidsgda srtpkdqkl reamaalrks aqdvqkfmda vnkksssqdl181 hkgtlsqmsg elskdgdliv smrilgkkrt ktwhkgtlia iqtvgpgkky kvkfdnkgks241 llsgnhiayd yhppadklyv gsrvvakykd gnqvwlyagi vaetpnvknk lrfliffddg301 yasyvtqsel ypicrplkkt wediediscr dfieeyvtay pnrpmvllks gqlikteweg361 twwksrveev dgslvrilfl ddkrcewiyr gstrlepmfs mktssasale kkqgqlrtrp421 mgavrskgp vvqytqdltg tgtqfkpvep pqptappapp fppapplspq agdsdlesql481 qsrkqvakk stsfrpgsvg sghssptspa lsenvsggkp ginqtyrspl gstasapaps541 alpappappv fhgmlerapa epsyrapmek lfylphvcsy tclsrvrpmr neqyrgknpl601 lvpllydfrr mtarrrvnrk mgfhviyktp cglclrtmqe ierylfetgc dflflemfcl661 dpyvlvdrkf qpykpfyyil ditygkedvp lscvneidtt pppqvayske ripgkgvfin721 tgpeflvgcd ckdgcrdksk cachqltiqa tactpggqin pnsgyqykrl eeclptgvye781 cnkrckcdpn mctnrlvqhg lqvrlqlfkt qnkgwgircl ddiakgsfvc iyagkiltdd841 fadkeglemg deyfanldhi esvenfkegy esdapcssds sgvdlkdqed gnsgtedpee901 snddssddnf ckdedfstss vwrsyatrrq trgqkengls ettskdshpp dlgpphipvp961 psipvggcnp psseetpknk vaswlscnsv seggfadsds hssfktnegg egraggsrme1021 aekastsglg ikdegdikqa kkedtddrnk msvvtessrn ygynpspvkp eglrrppskt1081 smhqsrrlma saqsnpddvl tlsssteseg esgtsrkpta gqtsatavds ddiqtissgs1141 egddfedkkn mtgpmkrqva vkstrgfalk sthgiaikst nmasvdkges apvrkntrqf1201 dgeescyii daklegnlgr ylnhscspnl fvqnvfvdth dlrfpwvaff askriragte1261 ltwdynyevg svegkellcc cgaiecrgrl l

Cross-Reactivity: Human

Description: The SET domain is a highly conserved,approximately 150-amino acid motif implicated in the modulation of chromatin structure. Itwas originally identified as part of a largerconserved region present in the Drosophila trithorax protein and was subsequentlyidentified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which theacronym SET isderived. Studies have suggested that the SET domain may be a signature of proteins thatmodulate transcriptionally active or repressed chromatin states through chromatinremodeling activities. ESET functions as a histone methyltransferase by methylation ofLys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetictranscriptional repression by recruiting HP1 proteins to methylated histones. ESET shows anuclear localization and is associated with non-pericentromeric regions of chromatin, andis excluded from nucleoli and islands ofcondensed chromatin. Although ESET is a widely expressed protein, it is highly expressedin the testis.

Characteristics: Synonyms: ESET, KMT1E, KIAA0067, Histone-lysine N-methyltransferase SETDB1, SET domainbifurcated 1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4,H3-K9-HMTase 4, Lysine N-methyltransferase 1E

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) andWestern blotting (1: 1,000-1: 2,500). Western blotting shows reactivity specific for human ESET detecting a band atapproximately 170 kDa. Specific band detection by Western blot is blocked by peptidecompetition by pre-incubating the antibody with the immunizing peptide prior to reactionwith the membrane. Reactivity in other immunoassays is unknown. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.17 mg/ml (by UV absorbance at 280 nm)

Purification: Affinity Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.

Storage: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product ifnot completely clear after standing at room temperature. This product is stable for onemonth at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis is shown using anti-ESET antibody to detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Publications

Schultz, Ayyanathan, Negorev et al.: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, 2002 (PubMed).