Cullin 1 antibody (C-Term)

Catalog No. ABIN117947

The Rabbit Polyclonal anti-Cullin 1 antibody has been validated for WB, IP, IHC (p) and EIA. It is suitable to detect Cullin 1 in samples from Human and Mouse.

Quick Overview for Cullin 1 antibody (C-Term) (ABIN117947)

Target: Cullin 1 (CUL1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Cullin 1 Antibody

Binding Specificity: AA 742-752, C-Term

Sequence: Human Cullin 1 (776 aa 89.6 kDa): 1 msstrsqnph glkqigldqi wddlragiqq vytrqsmaks rymelythvy nyctsvhqsn 61 qargagvpps kskkgqtpgg aqfvglelyk rlkeflknyl tnllkdgedl mdesvlkfyt 121 qqwedyrfss kvlngicayl nrhwvrrecd egrkgiyeiy slalvtwrdc lfrplnkqvt 181 navlklieke rngetintrl isgvvqsyve lglneddafa kgptltvyke sfesqfladt 241 erfytreste flqqnpvtey mkkaearlle eqrrvqvylh estqdelark ceqvliekhl 301 eifhtefqnl ldadknedlg rmynlvsriq dglgelkkll ethihnqgla aiekcgeaal 361 ndpkmyvqtv ldvhkkynal vmsafnndag fvaaldkacg rfinnnavtk maqssskspe 421 llarycdsll kkssknpeea eledtlnqvm vvfkyiedkd vfqkfyakml akrlvhqnsa 481 sddaeasmis klkqacgfey tsklqrmfqd igvskdlneq fkkhltnsep ldldfsiqvl 541 ssgswpfqqs ctfalpsele rsyqrftafy asrhsgrklt wlyqlskgel vtncfknryt 601 lqastfqmai llqynteday tvqqltdstq ikmdilaqvl qillkskllv ledenanvde 661 velkpdtlik lylgyknkkl rvninvpmkt eqkqeqetth knieedrkll iqaaivrimk 721 mrkvlkhqql lgevltqlss rfkprvpvik kcidilieke ylervdgekd tysyla

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.

Purification: Delipidation and defibrination.

Immunogen: Synthetic peptide corresponding to amino acids 742-752 of Human Cul1 (C-terminus) coupled to KLH.

Application Details

Application Notes: Western blot: This antibody reacts with human Cul1 (1/500-1/1,000, An 89.6 kDa bandcorresponding to human Cul1 is detected). Most cell lines expressing Cul1 can be used as apositive control. Immunoprecipitation: The antibody immunoprecipitates in vitro translated product andprotein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, butrather use 0.1 % SDS). ELISA (1/2,000-1/10,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85.0 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or at -20 °C for longer.

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: Cullin 1

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.Synonyms: CUL-1, CUL1, Cullin-1

Gene ID: 8454

NCBI Accession: NP_003583

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size