MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2) antibody

Antigen: MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2)

Synonyms: REV7, MAD2B, 2310033C13Rik, mad2l2-A, mad2B, MGC83968, mad2b, MGC69497, MAD2L2, MGC137331

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN117980

Additional Information

Alternative name: MAD2L2

UniProt: Q9UI95

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2. AA Sequence: Protein sequence: Human sequence, 211 amino acids, predicated MW 24. 3kDa. 1 MTTLTRQDLN FGQVVADVLC EFLEVAVHLI LYVREVYPVG IFQKRKKYNV PVQMSCHPEL61 NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF EITQPPLLSI SSDSLLSHVE121 QLLRAFILKI SVCDAVLDHN PPGCTFTVLV HTREAATRNM EKIQVIKDFP WILADEQDVH181 MHDPRLIPLK TMTSDILKMQ LYVEERAHKG S

Cross-Reactivity: Human

Description: MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents theonset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Characteristics: Synonyms: Mitotic spindle assembly checkpoint protein MAD2B, MAD2B, REV7

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 7,500-1: 30,000) againstthe immunizing peptide and by Western blotting (1: 500-1: 2,000) against HeLa whole celllysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibodyactivity can be blocked using a control peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.81 mg/ml (by UV absorbance at 280 nm)

Purification: Affinity Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.

Storage: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extendedstorage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Immunoblotting. Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.

Publications

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).

Nelson, Schloendorff, Blobel: "Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta." in: The Biochemical journal, Vol. 343 Pt 3, pp. 673-80, 2000 (PubMed).

Murakumo, Roth, Ishii et al.: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, 2000 (PubMed).