Integrin beta 2 antibody
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- Target See all Integrin beta 2 (ITGB2) Antibodies
- Integrin beta 2 (ITGB2)
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This Integrin beta 2 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody is specific for the common β2subunit of LFA-1 (CD11a/CD18), MAC-1 (CD11b/CD18) and P150,95 (CD11c/CD18) (2,3). These three β2 integrins, which function in cell-cell adhesion in the immune system, are also known as leukocyte integrins because their expression is limited to leukocytes (2,3).
- Purification
- Purified
- Immunogen
- Cell membrane glycoproteins derived from BW5147 cells (1)
- Clone
- C71-16
- Isotype
- IgG2a
- Top Product
- Discover our top product ITGB2 Primary Antibody
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- Application Notes
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IHC on acetone-fixed frozen sections. Immunoprecipitation. Flow cytometry (please see protocol for details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- PBS and 0.02 % sodium azide (NaN3)
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Integrin beta 2 (ITGB2)
- Alternative Name
- CD18 / ITGB2 (ITGB2 Products)
- Background
- CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
- Gene ID
- 16414
- NCBI Accession
- NP_032430
- UniProt
- P11835
- Pathways
- NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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