Integrin beta 2 antibody (ITGB2) (Biotin)

Details for Product anti-ITGB2 Antibody No. ABIN94007
Antigen
Reactivity
Human
433
170
107
21
19
9
6
4
4
3
3
3
3
2
2
Host
Mouse
275
179
101
2
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Integrin beta 2 antibody is conjugated to Biotin
74
60
42
20
11
8
7
6
5
5
5
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
338
235
143
126
86
57
56
51
39
39
10
10
8
7
6
1
1
1
1
1
Options
Immunogen Leukocytes of a patient suffering from a LGL-type leukemia.
Clone MEM-48
Isotype IgG1
Specificity The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:100. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Integrin beta 2 (ITGB2) antibody (Biotin) (ABIN94007) Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Co...
Image no. 2 for anti-Integrin beta 2 (ITGB2) antibody (Biotin) (ABIN94007) Fig. A Flow Cytometry analysis. Surface staining of human peripheral blood cells with...
Image no. 3 for anti-Integrin beta 2 (ITGB2) antibody (Biotin) (ABIN94007) Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-h...
Product cited in: Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009 (PubMed).

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991 (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Horejsí: "Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein." in: Folia biologica, Vol. 36, Issue 1, pp. 41-50, 1990 (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990 (PubMed).