Integrin beta 2 antibody (ITGB2)

Details for Product anti-ITGB2 Antibody No. ABIN457404
Target
Reactivity
Mouse
243
102
65
14
7
6
4
3
3
3
3
3
3
2
2
Host
Rat
122
119
54
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Integrin beta 2 antibody is un-conjugated
30
30
25
11
9
6
5
5
5
5
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
160
130
70
64
56
39
37
35
28
25
10
9
8
6
5
1
1
1
1
1
Options
Immunogen Murine cytotoxic T cell glycoproteins
Clone M18-2
Isotype IgG2a
Specificity The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit, beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Mouse
Purification Purified by protein-G affinity chromatography
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Target
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts.
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 μ,m filter sterilized.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Images
Image no. 1 for anti-Integrin beta 2 (ITGB2) antibody (ABIN457404) Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).
Product cited in: Cullere, Lauterbach, Tsuboi, Mayadas: "Neutrophil-selective CD18 silencing using RNA interference in vivo." in: Blood, Vol. 111, Issue 7, pp. 3591-8, 2008 (PubMed).

Varga, Balkow, Wild, Stadtbaeumer, Krummen, Rothoeft, Higuchi, Beissert, Wethmar, Scharffetter-Kochanek, Vestweber, Grabbe: "Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation." in: Blood, Vol. 109, Issue 2, pp. 661-9, 2007 (PubMed).

Watts, Beurskens, Martin-Padura, Ballantyne, Klickstein, Brenner, Lee: "Manifestations of inflammatory arthritis are critically dependent on LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 6, pp. 3668-75, 2005 (PubMed).

Barlow, Langston, Matthews, Chidlow, Kevil: "CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes." in: The American journal of pathology, Vol. 165, Issue 6, pp. 1849-52, 2004 (PubMed).

Sakurai, Taguchi, Anand, Ambati, Gragoudas, Miller, Adamis, Ambati: "Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 44, Issue 6, pp. 2743-9, 2003 (PubMed).

Abraham, Miller: "Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5193-201, 2001 (PubMed).

Avni, Pur, Yefenof, Baniyash: "Complement receptor 3 of macrophages is associated with galectin-1-like protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6151-8, 1998 (PubMed).

Zahalka, Naor: "Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness." in: International immunology, Vol. 6, Issue 6, pp. 917-24, 1994 (PubMed).

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993 (PubMed).

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983 (PubMed).