Sialoadhesin/CD169 antibody (Biotin)

Catalog No. ABIN118290

Cited in 1 publication. The Biotin-conjugated Rat Monoclonal anti-Sialoadhesin/CD169 antibody (Clone MOMA-1) (ABIN118290) specifically detects Sialoadhesin/CD169 in IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for Sialoadhesin/CD169 antibody (Biotin) (ABIN118290)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sialoadhesin/CD169 antibody is conjugated to Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MOMA-1

Product Details anti-Sialoadhesin/CD169 Antibody (Biotin)

Specificity: Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (Cat# BM4011). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF). The antigen detected by MOMA-1 is CD169, also known as Sialoadhesin. Antigen Distribution Isolated Cells: No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue Sections: Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.Antigen Distribution

Cross-Reactivity (Details): Species reactivity (tested):Mouse: Subpopulation of Mature Resident Tissue Macrophages.

Purification: Affinity Chromatography

Immunogen: Mouse lymph node tissue Remarks: The antigen is found intracellularly and on cell surface on metallophilic macrophages.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. Immunohistochemistry on frozen sections: approximate working dilution 0.5-1.0 m g/mL (1:400-1:800). Does not react on routinely processed paraffin sections. Suggested positive control: Mouse spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).

Concentration: 0.4 mg/mL

Buffer: Stock solutions contains PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.05 % Kathon as a preservative

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE!

References for anti-Sialoadhesin/CD169 antibody (Biotin) (ABIN118290)

Movita, Kreefft, Biesta, van Oudenaren, Leenen, Janssen, Boonstra: "Kupffer cells express a unique combination of phenotypic and functional characteristics compared with splenic and peritoneal macrophages." in: Journal of leukocyte biology, Vol. 92, Issue 4, pp. 723-33, (2012) (PubMed).

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: CD169 / SIGLEC1

Background: Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1

Gene ID: 20612

NCBI Accession: NP_035556

UniProt: Q62230