Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody

Antigen: Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1)

Synonyms: SN, CD169, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, SIGLEC-1, dJ1009E24.1, DKFZp667F058, Sn, Cd169, Siglec-1, SIGLEC1

Clonality: Monoclonal (MOMA-1)

Host: Rat

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Catalog no.: ABIN118292

Additional Information

Alternative name: CD169 / SIGLEC1

Immunogen: Mouse lymph node tissue

Isotype: IgG2a  (Matching secondary antibodies)

Clone: MOMA-1

Description: Alternate Names: Mouse Macrophage Marker.

Specificity: Clone MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by ER-TR 9. In addition , MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF). Antigen distribution: Isolated cells - No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue sections - Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine. The antigen is found intracellularly and on cell surface on metallophilic macrophages

Application Details

Application Notes: Immunohistochemistry on frozen sections: 2 ug/ml (1/200). Fixation: Acetone, Positive control: Mouse spleen. Flow cytometry. Does not react on routinely processed paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.4 mg IgG/ml

Buffer: PBS, pH 7.2, containing 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.

Storage: Original vial: 1 year from despatch at 2-8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing. Reconstitution: Restore by adding 0.5 ml distilled water..

Restrictions: For Research Use only

Publications

Publications: Kraal, Janse: "Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody." in: Immunology, Vol. 58, Issue 4, pp. 665-9, 1986 (PubMed).