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Interleukin 4 (IL4) antibody
| Antigen | Interleukin 4 (IL4) |
| Synonyms | BSF1, IL-4, BCGF1, BCGF-1, MGC79402, Il-4, IL4, Il4e12, IL-2, BSF-1 |
| Clonality | Monoclonal (BVD4-1D11) |
| Host |
 Alternatives Rat |
| Reactivity |
Alternatives Mouse (Murine) |
| Application |
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Capture), Flow Cytometry (FACS), Western Blotting (WB)
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3 references available |
| Catalog no. | ABIN118404 |
| Quantity | 0.5 mg (1.0 mg/ml) (Variants) |
| Price | 420.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | Interleukin-4 / IL4 |
| Swiss-Prot | P07750 |
| Immunogen | Recombinant Mouse IL-4. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | BVD4-1D11 |
| Description | Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells. |
| Specificity | This antibody is specific for Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of ABIN118403 for functional studies. |
Application Details
| Application Notes | Flow Cytometry: 1/10 - 1/50, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1ug/ml - 5ug/ml. Western Blot: 1ug/ml - 5ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Concentration | 1.0 mg/ml |
| Buffer | PBS, pH7.4 containing 0.09% Sodium Azide |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch. |
| Restrictions | For Research Use only |
Images
| Western blot analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE INTERLEUKIN-4 (ABIN118404) followed by F(ab')2 RABBIT ANTI RAT IgG:HRP |
Publications
| Publications |
Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).
Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed). Hochrein, OKeeffe, Luft et al.: "Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells." in: The Journal of experimental medicine, Vol. 192, Issue 6, pp. 823-33, 2000 (PubMed). |
Alternatives
Alternatives for antigen "Interleukin 4 (IL4)", type "Antibodies"
| Hosts | Mouse (26), Rat (25), Rabbit (20) |
| Reactivities | Human (32), Mouse (Murine) (21), Cow (Bovine) (6), Rat (Rattus) (6), Pig (Porcine) (3), Dog (Canine) (2), Goat (2), Sheep (Ovine) (2) |
| Applications | Western Blotting (WB) (38), Enzyme Immunoassay (EIA) (34), ELISA (20), Flow Cytometry (FACS) (16), Functional Studies (Func) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Immunofluorescence (IF) (6), Intracellular Staining (IS) (5), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (2), ELISA (Capture) (2), Immunoprecipitation (IP) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Neutralization (Neut) (1) |
| Conjugates | Biotin (11), PE (2), FITC (1), RPE (1) |
| Epitopes | Recombinant (1) |
