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Secondary Antibodies
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Rat anti-Mouse (Murine) Immunoglobulin M (IgM) (Heavy chain) Antibody (FITC)
Rat anti-Mouse (Murine) Immunoglobulin M (IgM) (Heavy chain) Antibody (FITC)
| Antigen |
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Epitope
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| Clonality |
Monoclonal (LO-MM-9) |
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Host
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Reactivity
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Human (113), Mouse (Murine) (99), Rat (Rattus) (51), Monkey (19), Chicken (18), Ferret (13), Sheep (Ovine) (8), Cat (Feline) (7), Cow (Bovine) (7), Dog (Canine) (7), Horse (Equine) (7), Rabbit (7), Goat (6), Pig (Porcine) (6), Hamster (4), Syrian Hamster (2), Donkey (1), Guinea Pig (1), Turkey (1)
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Conjugate
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FITC (66), HRP (64), Biotin (45), Alkaline Phosphatase (AP) (40), Texas Red (TR) (24), TRITC (20), Rhodamine (14), PE (8), DyLight 488 (6), Peroxidase (POD) (5), APC (4), Colloidal Gold (15 OD) (2), Colloidal Gold (50 OD) (2), RPE (2), SPRD (2), Cy5 (1), DyLight 405 (1), DyLight 594 (1), DyLight 680 (1), DyLight 800 (1), PE,Cy7 (1)
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Application
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Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
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| Catalog no. |
ABIN118420 |
| Quantity |
0.5 mg (1.0 mg/ml) |
| Price |
470.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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IgM (heavy chain)
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Immunogen
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Polyclonal and monoclonal mouse IgM. Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line
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Isotype
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IgG2a
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Clone
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LO-MM-9
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Specificity
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This antibody is directed against the mu chain of IgM.
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Application Notes
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Flow Cytometry: 5 ug/ml, use 5 ul of the suggested working dilution to label 10e6 cells in 100ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS, pH7.2 containing 0.09% Sodium Azide and 50% Glycerol
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.
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Research Area
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Secondary Antibodies
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Restrictions
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For Research Use only
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Publications
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Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c." in: European journal of immunology, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed).
Bazin, Cormont, De Clercq: "Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum." in: Journal of immunological methods, Vol. 71, Issue 1, pp. 9-16, 1984 (PubMed).
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Alternatives for antigen "Immunoglobulin M (IgM)", type "Secondary Antibodies"
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Hosts
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Goat (195), Rabbit (129), Mouse (35), Rat (13), Chicken (1)
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Reactivities
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Human (113), Mouse (Murine) (99), Rat (Rattus) (51), Monkey (19), Chicken (18), Ferret (13), Sheep (Ovine) (8), Cat (Feline) (7), Cow (Bovine) (7), Dog (Canine) (7), Horse (Equine) (7), Rabbit (7), Goat (6), Pig (Porcine) (6), Hamster (4), Syrian Hamster (2), Donkey (1), Guinea Pig (1), Turkey (1)
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Conjugates
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FITC (66), HRP (64), Biotin (45), Alkaline Phosphatase (AP) (40), Texas Red (TR) (24), TRITC (20), Rhodamine (14), PE (8), DyLight 488 (6), Peroxidase (POD) (5), APC (4), Colloidal Gold (15 OD) (2), Colloidal Gold (50 OD) (2), RPE (2), SPRD (2), Cy5 (1), DyLight 405 (1), DyLight 594 (1), DyLight 680 (1), DyLight 800 (1), PE,Cy7 (1)
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Epitopes
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Heavy chain (99), H&L (18), Fc5m (7), Whole (7), Fc5u (3), Fc- (2), Fc5u specific (1), Fcu (1)
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Alternatives 
