CD3 antibody (Biotin)

Catalog No. ABIN118477

Product Details anti-CD3 Antibody (Biotin)

Target: CD3

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the CD3 cell surface antigen, expressed by all mature peripheral blood T lymphocytes.

Purification: Affinity Chromatography on Protein G

Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.

Clone: UCHT1

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 ul of neat-1/10 diluted antibody to label 10e6 cells or 100ul of lysed whole peripheral blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Alternate names: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway