CD14 antibody (PE)

Catalog No. ABIN118511

There are 2+ publications for this product available. The Mouse Monoclonal anti-CD14 antibody is suitable to detect CD14 in samples from Human, Pig, Dog, Cow, Sheep, Rabbit and Goat. It has been validated for FACS.

Quick Overview for CD14 antibody (PE) (ABIN118511)

Target: CD14

Reactivity: Human, Pig, Dog, Cow, Sheep, Rabbit, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: Tük4

Product Details anti-CD14 Antibody (PE)

Purification: Affinity Chromatography on Protein G

Immunogen: CD14 Human

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

References for anti-CD14 antibody (PE) (ABIN118511)

Ogawa, Tsukahara, Imaoka, Nakanishi, Ushida, Inoue: "The effect of colostrum ingestion during the first 24 hours of life on early postnatal development of piglet immune systems." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 87, Issue 12, pp. 1511-1515, (2016) (PubMed).

Nishibayashi, Inoue, Harada, Watanabe, Makioka, Ushida: "RNA of Enterococcus faecalis Strain EC-12 Is a Major Component Inducing Interleukin-12 Production from Human Monocytic Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129806, (2016) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gene ID: 9606

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades