CD151 Molecule (Raph Blood Group) (CD151) antibody

Details for Product No. ABIN118882
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Antigen
Synonyms CD151, MGC83668, gp27, mer2, raph, sfa1, peta-3, tspan24, PETA-3, SFA-1, Tspan24, GP27, MER2, RAPH, SFA1, TSPAN24
Reactivity
Human
(68), (20), (20), (17), (17), (4), (2), (1)
Host
Mouse
(40), (37)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (7), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(46), (36), (27), (10), (10), (10), (7), (6), (1), (1), (1)
Pubmed 6 references available
Quantity 0.2 mg
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Catalog No. ABIN118882
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Immunogen Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Clone 11G5a
Isotype IgG1
Specificity This antibody recognizes the CD151 cell surface antigen, also known as PETA-3.
Purification Affinity Chromatography on Protein G
Alternative Name CD151 / TSPAN24
Background CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen.
Alternate names: GP27, Membrane glycoprotein SFA-1, PETA-3, PETA3, Platelet-endothelial tetraspan antigen 3, SFA-1, SFA1, Tetraspanin-24
Gene ID 977
NCBI Accession NP_001034579
UniProt P48509
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes ELISA. Flow cytometry: 1/50 - 1/100, use 10 ul of the suggested working dilution to label 10e6 cells or 100 ul of whole blood. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with heat mediated antigen retrieval in 0.01M citrate buffer. Recommended positive control: Tonsil/spleen. Western Blot. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions (ref.1).
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-CD151 Molecule (Raph Blood Group) (CD151) antibody Staining of human peripheral blood platelets with Mouse anti Human CD151 (ABIN118882).
Background publications Yauch, Berditchevski, Harler et al.: "Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration." in: Molecular biology of the cell, Vol. 9, Issue 10, pp. 2751-65, 1998 (PubMed).

Karamatic Crew, Burton, Kagan et al.: "CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin." in: Blood, Vol. 104, Issue 8, pp. 2217-23, 2004 (PubMed).

Zheng, Liu: "CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation." in: Acta pharmacologica Sinica, Vol. 28, Issue 1, pp. 66-72, 2006 (PubMed).

Zheng, Liu: "CD151 gene delivery activates PI3K/Akt pathway and promotes neovascularization after myocardial infarction in rats." in: Molecular medicine (Cambridge, Mass.), Vol. 12, Issue 9-10, pp. 214-20, 2007 (PubMed).

Hasegawa, Furuya, Kasuya et al.: "CD151 dynamics in carcinoma-stroma interaction: integrin expression, adhesion strength and proteolytic activity." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 9, pp. 882-92, 2007 (PubMed).

Franco, Muratori, Corso et al.: "The tetraspanin CD151 is required for Met-dependent signaling and tumor cell growth." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 38756-64, 2010 (PubMed).

Validation Images
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