CD151 antibody (AA 113-175)

Catalog No. ABIN5518817

The Rabbit Polyclonal anti-CD151 antibody (ABIN5518817) specifically detects CD151 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CD151 antibody (AA 113-175) (ABIN5518817)

Target: CD151

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD151 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD151 Antibody

Binding Specificity: AA 113-175

Purpose: Rabbit IgG polyclonal antibody for CD151 antigen(CD151) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for antigen detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name:  Molecule (Raph blood group)
Protein Name: antigen

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD151 recombinant protein (Position: A113-A175). Human CD151 shares 88.7% amino acid (aa) sequence identity with both mouse and rat CD151.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CD151

Target: CD151

Alternative Name: CD151

Background: CD151 Molecule (Raph blood group), also known as CD151 (Cluster of Differentiation 151), is a human gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.

Synonyms: CD151 | CD151 antigen | CD151 Molecule | GP27 | MER2 | PETA-3 | PETA3 | RAPH | SFA 1 | SFA1 | Tetraspanin-24 | Tspan-24 | TSPAN24 | P48509

Gene ID: 977

UniProt: P48509