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CD151 antibody

CD151 Reactivity: Human WB, FACS, IHC (p), IP, IHC (fro), EIA Host: Mouse Monoclonal 11G5a unconjugated
Catalog No. ABIN118883
  • Target See all CD151 Antibodies
    CD151
    Reactivity
    • 65
    • 33
    • 16
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Host
    • 50
    • 17
    Mouse
    Clonality
    • 50
    • 17
    Monoclonal
    Conjugate
    • 27
    • 5
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD151 antibody is un-conjugated
    Application
    • 40
    • 32
    • 22
    • 15
    • 13
    • 13
    • 13
    • 8
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody recognizes the CD151 cell surface antigen, also known as PETA-3.
    Purification
    Affinity Chromatography on Protein G
    Immunogen
    Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
    Clone
    11G5a
    Isotype
    IgG1
    Top Product
    Discover our top product CD151 Primary Antibody
  • Application Notes
    ELISA. Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with heat mediated antigen retrieval in 0.01M citrate buffer. Recommended positive control: Tonsil/spleen. Western Blot. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions .
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD151
    Alternative Name
    CD151 / TSPAN24 (CD151 Products)
    Synonyms
    CD151 antibody, MGC83668 antibody, gp27 antibody, mer2 antibody, raph antibody, sfa1 antibody, peta-3 antibody, tspan24 antibody, PETA-3 antibody, SFA-1 antibody, Tspan24 antibody, GP27 antibody, MER2 antibody, RAPH antibody, SFA1 antibody, TSPAN24 antibody, CD151 molecule antibody, CD151 molecule (Raph blood group) L homeolog antibody, CD151 molecule (Raph blood group) antibody, CD151 antigen antibody, CD151 antibody, cd151.L antibody, cd151 antibody, Cd151 antibody
    Background
    CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen.Synonyms: GP27, Membrane glycoprotein SFA-1, PETA-3, PETA3, Platelet-endothelial tetraspan antigen 3, SFA-1, SFA1, Tetraspanin-24
    Gene ID
    977
    NCBI Accession
    NP_001034579
    UniProt
    P48509
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