Fibroblasts, Epithelial Cells antibody

Catalog No. ABIN118888

This Mouse Monoclonal antibody specifically detects Fibroblasts, Epithelial Cells in FACS, IP and IHC (fro). It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Fibroblasts, Epithelial Cells antibody (ABIN118888)

Target: Fibroblasts, Epithelial Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: D7-FIB

Product Details

Specificity: SM1214P recognises a 112 kDa molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (Van Osch et al., 2001).

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: use 10 μL of a 1:50 - 1:200 dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on the KG1 cell line. Immunohistochemistry on frozen tissues or cells (1:100): use ice-cold methanol for 5 min as fixative. Does not work on paraffin sections. Can also be used for immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References

Nazareth, Broderick, Simpson-Abelson, Kelleher, Yokota, Bankert: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).

Target Details

Target: Fibroblasts, Epithelial Cells

Alternative Name: Fibroblasts / Epithelial Cells

Background: A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.Synonyms: Fibroblast Marker, Fibroblasten