Fibroblasts, Epithelial Cells antibody (PE)

Catalog No. ABIN1819650

The PE-conjugated Mouse Monoclonal anti-Fibroblasts, Epithelial Cells antibody (Clone D7-FIB) (ABIN1819650) specifically detects Fibroblasts, Epithelial Cells in FACS, IP, IHC (fro) and IHC. The antibody is reactive with Human samples.

Quick Overview for Fibroblasts, Epithelial Cells antibody (PE) (ABIN1819650)

Target: Fibroblasts, Epithelial Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Clone: D7-FIB

Product Details

Specificity: Recognizes a ~112kD molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies, has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. Is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes. Is routinely tested in flow cytometry on the KG1 cell line.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G purified

Immunogen: Human foreskin fibroblasts.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1 - 1:10), IHC, IHC-Fr, IP

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA, 5 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details

Target: Fibroblasts, Epithelial Cells

Alternative Name: Fibroblasts + Epithelial Cells

Background: This antibody detects: Fibroblasts + Epithelial Cells