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HLA Class I ABC antibody

Details for Product No. ABIN118900
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Antigen
Reactivity
Cow (Bovine), Cat (Feline), Human, Monkey, Primate
(23), (17), (8), (6), (5), (1), (1), (1)
Host
Mouse
(30), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (5), (4), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
(27), (10), (7), (5), (4), (3), (2), (2), (2), (1), (1)
Pubmed 10 references available
Quantity 0.2 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN118900
500.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified human tonsil lymphocyte membranes
Clone W6-32
Isotype IgG2a
Specificity Antibody SM1222P recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (7). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
Purification Affinity Chromatography on Protein G
Background Alternate names: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I
Research Area Major Histocompatibility Complex (MHC)
Application Notes ELISA. Immunoprecipitation. Immunohistochemistry on frozen sections. (Does not work on paraffin sections). Flow cytometry (1:50 - 1:100): use 10 ul of diluted antibody to label 1x10e6 cells or 100 ul whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09% Sodium Azide as a preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-HLA Class I ABC antibody Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC (ABIN118900).
anti-HLA Class I ABC antibody (2) HLA Class 1 staining (ABIN118900) on Human tonsil. Cryostat Section.
Background publications Barnstable, Bodmer, Brown et al.: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, 1978 (PubMed).

Tanabe, Sekimata, Ferrone et al.: "Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 10, pp. 3202-9, 1992 (PubMed).

Neefjes, Hensen, de Kroon et al.: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." in: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, 1986 (PubMed).

Stern, Beresford, Friedman et al.: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987 (PubMed).

Verbeek, Westphal, Ruiter et al.: "T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5876-84, 1995 (PubMed).

Jacobsen, Aasted, Broe et al.: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994 (PubMed).

Yoshino, Ami, Terao et al.: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).

Ishitani, Sageshima, Lee et al.: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003 (PubMed).

General Burrone, Milstein: "Control of HLA-A,B,C synthesis and expression in interferon-treated cells." in: The EMBO journal, Vol. 1, Issue 3, pp. 345-9, 1984 (PubMed).

Stern, Goldman, Hatasaka et al.: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, 2008 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN118900
500.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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