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HLA Class I ABC antibody

Details for Product No. ABIN118900, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine), Cat (Feline), Human, Monkey, Primate
15
9
8
6
5
3
1
1
1
Host
Mouse
14
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
2
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
13
4
4
2
2
2
1
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Immunogen Purified human tonsil lymphocyte membranes
Clone W6-32
Isotype IgG2a
No Cross-Reactivity Goat, Dog (Canine), Guinea Pig, Rabbit, Mouse (Murine), Chicken, Amphibian
Cross-Reactivity (Details) Species reactivity (tested):Human, Cynomolgus monkey, Rhesus monkey, Chimpanzee, Macaque, Bovine, Baboon, Gorilla, Shrew.
Purification Affinity Chromatography on Protein G
Background The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I
Research Area Major Histocompatibility Complex (MHC)
Pathways
Application Notes ELISA. Immunoprecipitation. Immunohistochemistry on frozen sections. (Does not work on paraffin sections). Flow cytometry (1:50 - 1:100): use 10 μL of diluted antibody to label 1x10^6 cells or 100 μL whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-HLA Class I ABC antibody (ABIN118900) Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC (ABIN118...
 image for anti-HLA Class I ABC antibody (ABIN118900) HLA Class 1 staining (ABIN118900) on Human tonsil. Cryostat Section.
Product cited in: Stern, Goldman, Hatasaka et al.: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, 2008 (PubMed).

Mayr, Kofler, Büning et al.: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." in: Blood, Vol. 106, Issue 9, pp. 3223-6, 2005 (PubMed).

Background publications Ishitani, Sageshima, Lee et al.: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003 (PubMed).

Yoshino, Ami, Terao et al.: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).

Jacobsen, Aasted, Broe et al.: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994 (PubMed).

Verbeek, Westphal, Ruiter et al.: "T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5876-84, 1995 (PubMed).

Burrone, Milstein: "Control of HLA-A,B,C synthesis and expression in interferon-treated cells." in: The EMBO journal, Vol. 1, Issue 3, pp. 345-9, 1984 (PubMed).

Stern, Beresford, Friedman et al.: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987 (PubMed).

Neefjes, Hensen, de Kroon et al.: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." in: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, 1986 (PubMed).

Tanabe, Sekimata, Ferrone et al.: "Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 10, pp. 3202-9, 1992 (PubMed).

Barnstable, Bodmer, Brown et al.: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, 1978 (PubMed).