Keratin 14 (KRT14) antibody

Antigen: Keratin 14 (KRT14)

Synonyms: K14, NFJ, CK14, EBS3, EBS4, Krt1-14, AI626930, Krt-1.14, Krt14l, Krt14, Ka14, KRT14, ouro2

Clonality: Monoclonal (LL002)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN118965

Additional Information

Alternative name: Cytokeratin 14

UniProt: P02533

Immunogen: Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin

Cross-Reactivity: Human

Isotype: IgG3

Clone: LL002

Description: Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Alternate Names: Cytokeratin 14, CK-14, CK14, K-14, Keratin-14, Keratin 14, K14, Keratin Type I Cytoskeletal 14, Cytokeratin14, Keratin14.

Application Details

Application Notes: Flow Cytometry: Use 10 ul of 1/100 diluted antibody to label 10e6 cells in 100 ul (Membrane permeabilisation is required). Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/200): This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer is recommended for this purpose, Enzyme digestion is not recommended. Recommended Positive Control: Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer

Restrictions: For Research Use only

Publications

Purkis, Steel, Mackenzie et al.: "Antibody markers of basal cells in complex epithelia." in: Journal of cell science, Vol. 97 ( Pt 1), pp. 39-50, 1991 (PubMed).

Lane, Alexander: "Use of keratin antibodies in tumor diagnosis." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 165-79, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).