TSH receptor antibody

Catalog No. ABIN119039

Product Details anti-TSH receptor Antibody

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TSH receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: 2C11 recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors. This antibody recognises the mutant TSH receptor known as I167N as well as the wild type molecule.

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant Human TSH receptor. Spleen cells from immunised BALB/c mice were fused with cells of the NS1/Ag4.1 mouse myeloma cell line.

Clone: 2C11

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. ELISA. Immunoprecipitation: 5-10 μg/mL. Western Blot: 5-10 μg/mL. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TSH receptor

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Alternative Name: Thyrotropin Receptor / TSHR (TSHR Products)

Synonyms: CHNG1 antibody, LGR3 antibody, hTSHR-I antibody, AI481368 antibody, hypothroid antibody, hyt antibody, pet antibody, TSHRA antibody, TSH-R antibody, thyroid stimulating hormone receptor antibody, tshr antibody, TSHR antibody, Tshr antibody

Background: The thyroid stimulating hormone (TSH) receptor (otherwise known as the thyrotropin receptor) is an important molecule in controlling the growth and function of the normal thyroid.Synonyms: LGR3, TSH-R, TSH-receptor, Thyroid-stimulating hormone receptor

Gene ID: 7253

NCBI Accession: NP_000360

UniProt: P16473

Pathways: Thyroid Hormone Synthesis