HLA-A2 antibody

Catalog No. ABIN119575

This Mouse Monoclonal antibody specifically detects in FACS, IP, IHC (fro) and Func. It exhibits reactivity toward Human.

Quick Overview for HLA-A2 antibody (ABIN119575)

Target: HLA-A2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: BB7-2

Product Details

Specificity: This antibody recognises the human HLA-A2 histocompatibility antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. BB7.2 may be used for the Flow cytometric detection of HLA-A2 expression and has also been used for Immunoaffinity purification of HLA-A2 Molecules. Functionally the antibody is reported to inhibit MHC restricted cellular cytotoxicity.

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Less than 0.01EU/μg

Immunogen: Papain solubilised HLA-A2. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry (10 μg/mL): use 10 μL of of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional assay (Inhibition of MHC resticted cytotoxicity): 5 - 10 μg/mL. Immunohistochemistry on frozen sections. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without stabilizer or preservative

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details

Target: HLA-A2

Alternative Name: HLA Class I A-2 alpha / HLA-A2

Target Type: Influenza Protein

Background: Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2

Gene ID: 100507703

NCBI Accession: XP_003119274

UniProt: P01892