Cytokeratin 19 antibody

Catalog No. ABIN119586

This anti-Cytokeratin 19 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 19 in WB, IF, FACS, IHC (p), IP and IHC (fro). Suitable for Human.

Quick Overview for Cytokeratin 19 antibody (ABIN119586)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BA-17

Product Details anti-Cytokeratin 19 Antibody

Specificity: The antibody reacts with Cytokeratin 19 (40 kDa) in Human Tissue. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with Cytokeratin 7.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Affinity Chromatography.

Immunogen: Mammary organoids

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/100, this product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Recommended Positive Control Tissue: Normal breast. Western blotting. Flow Cytometry. Immunoprecipitation. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19

Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19

Gene ID: 3880

NCBI Accession: NP_002267

UniProt: P08727