NKG2A/C/E antibody (FITC)

Catalog No. ABIN119952

The FITC-conjugated Rat Monoclonal anti-NKG2A/C/E antibody (Clone 20d5) (ABIN119952) specifically detects NKG2A/C/E in FACS. The antibody is reactive with Mouse samples.

Quick Overview for NKG2A/C/E antibody (FITC) (ABIN119952)

Target: NKG2A/C/E (KLRC1/2/3) (Killer Cell Lectin-Like Receptor Subfamily C, Member 1/2/3 (KLRC1/2/3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This NKG2A/C/E antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 20d5

Product Details anti-NKG2A/C/E Antibody (FITC)

Purification: Affinity chromatography on Protein G

Immunogen: CHO transfected cells expressing the B6 allele of NKG2A. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NKG2A/C/E

Target: NKG2A/C/E (KLRC1/2/3) (Killer Cell Lectin-Like Receptor Subfamily C, Member 1/2/3 (KLRC1/2/3))

Alternative Name: NKG2A/C/E

Background: In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.Synonyms: CD159a, CD159c, CD159e, NKG2 lectin-like family