Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (FITC)

Details for Product No. ABIN119996
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Antigen
Synonyms ITGA5, Cd49e, Fnra, VLA5, CD49e, FNRA, VLA5A, Itga5_retired
Reactivity
Mouse (Murine)
(111), (107), (35), (13), (12), (3), (1)
Host
Rat
(62), (52), (45), (37), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(31), (19), (13), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(130), (71), (47), (41), (35), (27), (15), (13), (10), (10), (6), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN119996
253.00 $
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Immunogen Mouse MC/9 cell line. Remarks: Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
Clone MFR5 (5H10)
Isotype IgG2a
Specificity This antibody recognises Integrin alpha 5 (CD49e), which associates with the beta 1 integrin (CD29) to form the fibronectin receptor CD49e/CD29 (VLA-5). Clone MFR5(5H10) has been reported to block CD49e mediated function. We recommend the use of SM2100LE for this purpose.
Purification Affinity Chromatography on Protein G
Alternative Name CD49e / ITGA5
Background Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.
Alternate names: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
Gene ID 10090
UniProt P11688
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (FITC) Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD49e: FITC (ABIN119996).
Background publications Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).

van der Loo, Xiao, McMillin et al.: "VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin." in: The Journal of clinical investigation, Vol. 102, Issue 5, pp. 1051-61, 1998 (PubMed).

Cukierman, Pankov, Stevens et al.: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001 (PubMed).

Validation Images
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