Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2 (EMR2) antibody

Antigen: Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2 (EMR2)

Synonyms: CD312, DKFZp781B135, EMR2

Clonality: Monoclonal (2A1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN120169

Additional Information

Alternative name: CD312 / EMR2

Swiss-Prot: Q9UHX3

Immunogen: NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2A1

Description: EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Alternate Names: EGF-Like Module-Containing Mucin-Like Receptor 2, EMR-2, EMR2, EGF-Like Module-Containing Mucin-Like Receptor 2, EGF-like Module EMR2.

Specificity: This antibody recognises EMR2, a member of the epidermal growth factor-seven transmembrane (EGFTM7) family of proteins, which is closely related to CD97. Clone 2A1 specifically recognises the stalk region of EMR2.

Application Details

Application Notes: Flow Cytometry: Use 10 ul of 1/50-1/200 diluetd antibody to label 10e6 cells in 100 ul. Immunoprecipitation. Western Blot. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH7.4 containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD312 (ABIN120169).

Publications

Publications: Kwakkenbos, Kop, Stacey et al.: "The EGF-TM7 family: a postgenomic view." in: Immunogenetics, Vol. 55, Issue 10, pp. 655-66, 2004 (PubMed).