KLRK1 antibody

Catalog No. ABIN120293

Product Details anti-KLRK1 Antibody

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLRK1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6).

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.

Clone: 1D11

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/200, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01EU/mg

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE!

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: CD314 / KLRK1 (KLRK1 Products)

Synonyms: KLRK1 antibody, NKG2D antibody, CD314 antibody, D12S2489E antibody, KLR antibody, NKG2-D antibody, D6H12S2489E antibody, Nkg2d antibody, Nkrp2 antibody, killer cell lectin like receptor K1 antibody, killer cell lectin-like receptor subfamily K, member 1 antibody, NKG2D protein antibody, KLRK1 antibody, Klrk1 antibody, NKG2D antibody

Background: CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells.Synonyms: D12S2489E, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D

Gene ID: 100528032

NCBI Accession: NP_001186734

UniProt: P26718

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process