This antibody is specific for CD314 also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6). We recommend the use of SM2260LE for such studies.
Purification
Affinity chromatography on Protein G
Immunogen
NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
Flow Cytometry (Neat-1/10): Use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Target
KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells.Synonyms: D12S2489E, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D