CD4 antibody (Domain 1) (FITC)
Quick Overview for CD4 antibody (Domain 1) (FITC) (ABIN120375)
Target
See all CD4 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- Domain 1
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Purification
- Affinity Chromatography on Protein G.
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Immunogen
- Rat thymocyte membrane preperation.
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Isotype
- IgG1
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Application Notes
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Flow cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded Sections. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine- paraformaldehyde) (see Ref. 3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.1 mg/mL
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Buffer
- PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD4
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Alternative Name
- CD4
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Background
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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Gene ID
- 10116
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UniProt
- P05540
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Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Target
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