CD55 antibody
| Antigen |
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| Clonality |
Monoclonal (MEM-118) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN120605 |
| Quantity |
0.1 mg (1.0 mg/ml) (Variants) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CD55 / DAF
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Swiss-Prot
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P08174
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Immunogen
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HPB-ALL human T cell line
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Isotype
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IgM (Matching secondary antibodies)
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Clone
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MEM-118
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Description
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CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. Alternate Names: DAF-GPI, CD55, Cd55a, DAF, DAF1, CR, DAF-1, Complement decay-accelerating factor, GPI- anchored precursor.
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Specificity
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The antibody recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.
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Application Notes
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Flow Cytometry. Immunoprecipitation. Immunohistochemistry (paraffin sections) Recommended dilution: 10 m g/ml Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4.
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Storage
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Store the antibody at 2 - 8 °C. Do not freeze. Shelf Life: one year from despatch.
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Restrictions
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For Research Use only
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Publications
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Cinek, Horejsi: "The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2262-70, 1992 (PubMed).
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Alternatives for antigen "CD55", type "Antibodies"
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Hosts
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Mouse (16), Rabbit (1)
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Reactivities
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Human (17), Primate (3)
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Applications
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Flow Cytometry (FACS) (16), Western Blotting (WB) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunoprecipitation (IP) (3), Enzyme Immunoassay (EIA) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Conjugates
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PE (3), FITC (2), BIMA (1), Biotin (1), SPRD (1)
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