CD55 antibody

Catalog No. ABIN6939225

The Mouse Monoclonal anti-CD55 antibody is suitable to detect CD55 in samples from Human. It has been validated for FACS and IF.

Quick Overview for CD55 antibody (ABIN6939225)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: F4-29D9

Product Details anti-CD55 Antibody

Purpose: CD55 / Decay Accelerating Factor (DAF) Antibody

Specificity: Recognizes a single chain glycoprotein of 70 kDa, identified as CD55. This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human umbilical vein endothelial cells (HUVEC)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Jurkat, HUT-78, K562, YT, U937, MG63 and human lymphocytes. Human lymph nodes and tonsils.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background:

CD55, CD55 Cromer blood group system, Complement decay-accelerating factor, CROM, DAF, Decay Accelerating Factor for Complement, Decay accelerating factor GPI-form, Decay accelerating factor soluble-form, GPI-DAF

HUT-78, Jurkat, K562, MG63 and human lymphocytes. Human lymph nodes and tonsils., U937, YT

Molecular Weight: 70kDa

Gene ID: 1604, 126517

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity