SLC9A3R1 antibody

Catalog No. ABIN120629

The Mouse Monoclonal anti-SLC9A3R1 antibody is suitable to detect SLC9A3R1 in samples from Human. It has been validated for WB, IP and IHC (p).

Quick Overview for SLC9A3R1 antibody (ABIN120629)

Target: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC9A3R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EBP10

Product Details anti-SLC9A3R1 Antibody

Specificity: The antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.

Purification: Affinity Chromatography on Protein A

Immunogen: Bacterially produced recombinant full-length human NHERF

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation. Western Blotting: 2 m g/mL, 60 min on vertical incubator. Positive control: RAJI human lymphoma cell lysate. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. 10 % separating SDS-PAGE gel. Immunohistochemistry (paraffin sections): Recommended dilution: 5 m g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for SLC9A3R1

Target: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Alternative Name: SLC9A3R1 / NHERF

Background: NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.Synonyms: EBP50, Ezrin-radixin-moesin-binding phosphoprotein 50, NHERF-1, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1

Gene ID: 9368

UniProt: O14745

Pathways: Proton Transport, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome