SIT1 antibody

Catalog No. ABIN120658

This Mouse Monoclonal antibody specifically detects SIT1 in WB, IP and FACS. It exhibits reactivity toward Human.

Quick Overview for SIT1 antibody (ABIN120658)

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: SIT-01

Product Details anti-SIT1 Antibody

Specificity: The antibody reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.

Characteristics: Synonyms: Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembraneadapter protein, Suppression-inducing transmembrane adapter 1

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Bacterially produced recombinant intracellular fragment of human SIT.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (intracellular staining). Immunoprecipitation. Western Blotting: Western blot analysis demonstrated that SIT is expressed as anapproximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidasetreatment.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SIT1

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Alternative Name: SIT1 / SIT

Background: SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.Synonyms: SHP2-interacting transmembrane adapter protein, Signaling threshold-regulating transmembrane adapter 1, Suppression-inducing transmembrane adapter 1

Gene ID: 27240

UniProt: Q9Y3P8