SIT1 antibody (Signaling threshold Regulating Transmembrane Adaptor 1)

Details for Product anti-SIT1 Antibody No. ABIN94465
Antigen
Reactivity
Human
38
3
Host
Mouse
21
13
7
Clonality (Clone)
Monoclonal ()
Conjugate
This SIT1 antibody is un-conjugated
9
3
3
3
3
3
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
32
22
12
11
3
1
1
1
Options
Immunogen Bacterially produced recombinant intracellular fragment of human SIT.
Clone SIT-01
Isotype IgG1
Specificity The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name SIT (SIT1 Antibody Abstract)
Background SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1) antibody (ABIN94465) Western blot of human Jurkat T cell line
Product cited in: Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, 2005 (PubMed).

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004 (PubMed).