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SIT1 antibody

SIT1 Reactivity: Human WB, IP, FACS Host: Mouse Monoclonal SIT-01 unconjugated
Catalog No. ABIN94465
  • Target See all SIT1 Antibodies
    SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))
    Reactivity
    • 21
    • 2
    Human
    Host
    • 17
    • 5
    • 1
    Mouse
    Clonality
    • 18
    • 5
    Monoclonal
    Conjugate
    • 10
    • 5
    • 2
    • 2
    • 2
    • 2
    This SIT1 antibody is un-conjugated
    Application
    • 20
    • 14
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)
    Specificity
    The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Bacterially produced recombinant intracellular fragment of human SIT.
    Clone
    SIT-01
    Isotype
    IgG1
  • Application Notes
    Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
    Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

    Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, (2004) (PubMed).

  • Target
    SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))
    Alternative Name
    SIT (SIT1 Products)
    Synonyms
    SIT1 antibody, RP11-331F9.5 antibody, SIT antibody, SIT-R antibody, Sit antibody, signaling threshold regulating transmembrane adaptor 1 antibody, suppression inducing transmembrane adaptor 1 antibody, SIT1 antibody, Sit1 antibody
    Background
    Signaling threshold regulating transmembrane adapt,SIT is a 21 kDa transmembrane adaptor protein expressed exclusively in lymphoid organs. It acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.,SIT1
    Gene ID
    27240
    UniProt
    Q9Y3P8
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