MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody

Catalog No. ABIN120723

Cited in 1 publication. The Mouse Monoclonal anti-MHC Class I RT1.Aa alpha-Chain antibody (Clone OX-18) (ABIN120723) specifically detects MHC Class I RT1.Aa alpha-Chain in FACS, IHC (fro) and IP. The antibody is reactive with Rat samples.

Quick Overview for MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody (ABIN120723)

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: OX-18

Product Details

Purification: Affinity chromatography on Protein G

Immunogen: Rat spleen glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line

Isotype: IgG1

Application Details

Application Notes: ELISA. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References

Sakurada, Osawa, Araki: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).

Target Details

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Alternative Name: MHC Class I (RT1Aa)

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain