MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody

Catalog No. ABIN120722

Product Details

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Purification: Protein G affinity chromatography

Immunogen: Rat spleen glycoproteins

Clone: OX-18

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections: Acetone fixation is recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable for immunohistochemistry on paraffin sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References

Sakurada, Osawa, Araki: "[Aortic root replacement and pulmonary vein isolation, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 2, pp. 153-6, (2008) (PubMed).

Target Details

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Alternative Name: MHC Class I (RT1Aa) (RT1.AA Products)

Synonyms: RTA antibody, Rt1.aa antibody, RT1 class I, locus A antibody, RT1-A antibody

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain