LCP2 antibody (AA 216-434)

Catalog No. ABIN120741

The Mouse Monoclonal anti-LCP2 antibody (Clone SLP-76-03) (ABIN120741) specifically detects LCP2 in WB and IHC (p). The antibody is reactive with Human, Mouse and Pig samples.

Quick Overview for LCP2 antibody (AA 216-434) (ABIN120741)

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Reactivity: Human, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This LCP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SLP-76-03

Product Details anti-LCP2 Antibody

Binding Specificity: AA 216-434

Specificity: The antibody reacts with SLP76, a 76 kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils, in B cells, however, it is replaced by SLP65.

Purification: Protein G affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: A fusion protein representing amino acids 216-434 of human SLP-76

Isotype: IgG2b

Application Details

Application Notes: Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for LCP2

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Alternative Name: LCP2

Background: SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets, B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-g. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.Synonyms: LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76

Gene ID: 3937

UniProt: Q13094

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway