SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) antibody

Antigen: SET Domain Containing (Lysine Methyltransferase) 7 (SETD7)

Synonyms: KMT7, Set7, H3K4MT, Set7/9, mKIAA1717, 1600028F23Rik, SET7, SET9, SET7/9, FLJ21193, KIAA1717

Clonality: Monoclonal (S4E5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121150

Additional Information

Alternative name: SETD7 / SET9

UniProt: Q8WTS6

Immunogen: Recombinant human SET7/9 protein

Cross-Reactivity: Human

Isotype: IgG2b

Clone: S4E5

Description: Set7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase. Alternate Names: Histone Methyltransferase, SET-7, KMT7, SET7/9, KIAA1717, SET9.

Application Details

Application Notes: ELISA. Western blot (1:250 - 1:2,000), recommended starting dilution : 1:1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4, containing 0.09% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Immunoblot analysis.,.Recombinant SET7/9 was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-SET7/9(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicated SET7/9(~50Da).

Publications

Nishioka, Chuikov, Sarma et al.: "Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation." in: Genes & development, Vol. 16, Issue 4, pp. 479-89, 2002 (PubMed).