SETD7 antibody (AA 107-366)

Catalog No. ABIN7193392

This Mouse Monoclonal antibody specifically detects SETD7 in WB, ELISA and FACS. It exhibits reactivity toward Human, Rat and Monkey.

Quick Overview for SETD7 antibody (AA 107-366) (ABIN7193392)

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Reactivity: Human, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SETD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 1B3B8

Product Details anti-SETD7 Antibody

Binding Specificity: AA 107-366

Purpose: SETD7 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human SETD7 (AA: 107-366) expressed in E. Coli.

Isotype: IgG2a

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for SETD7

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Alternative Name: SETD7

Background:

Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

Molecular Weight: 40.7 kDa

UniProt: Q8WTS6