Interferon Regulatory Factor 5 (IRF5) antibody

Antigen: Interferon Regulatory Factor 5 (IRF5)

Synonyms: SLEB10, mirf5, AW491843, MGC76986, zgc:76986, im:7155364, IRF5, irf5

Clonality: Monoclonal (10T1)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121152

Additional Information

Alternative name: IRF5

UniProt: Q13568

Immunogen: Recombinant human IRF-5 (176-240 aa) purified from E. coli

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 10T1

Description: IRF-5 is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs(IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines. Alternate Names: Interferon Regulatory Factor 5, IRF-5.

Application Details

Application Notes: ELISA. Western blot (1/500 - 1/1,000). Immunoprecipitation. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS buffer pH 7.4 with 0.02% Thimerosal as preservative

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines

Restrictions: For Research Use only

Images

Western blot analysis The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Publications

Barnes, Moore, Pitha: "Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23382-90, 2001 (PubMed).

Barnes, Kellum, Pinder et al.: "Interferon regulatory factor 5, a novel mediator of cell cycle arrest and cell death." in: Cancer research, Vol. 63, Issue 19, pp. 6424-31, 2003 (PubMed).

Ning, Huye, Pagano: "Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit." in: Journal of virology, Vol. 79, Issue 18, pp. 11671-6, 2005 (PubMed).