MRGPRC antibody (N-Term)

Catalog No. ABIN122228

The Rabbit Polyclonal anti-MRGPRC antibody is suitable to detect MRGPRC in samples from Human. It has been validated for WB, IHC (p) and IF.

Quick Overview for MRGPRC antibody (N-Term) (ABIN122228)

Target: MRGPRC (MAS-Related G-Protein Coupled Receptor, Member C (MRGPRC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRGPRC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-MRGPRC Antibody

Binding Specificity: N-Term

Specificity: Reacts with G protein-coupled receptor MRGX1 (SNSR3/SNSR4) at N-term.BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MRGPRX3 (71 %).

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Western Blot. Immunocytochemistry. Immunohistochemistry with formalin fixed paraffin embedded tissues: 12 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for MRGPRC

Target: MRGPRC (MAS-Related G-Protein Coupled Receptor, Member C (MRGPRC))

Alternative Name: MRGPRC

Background: MRGX1 belongs to the Opioid Receptor subfamily because this receptor is activated by products of proenkephalin A, an opioid peptide precursor. MRGX1 has been reported exclusively in dorsal root ganglion. ESTs for MRGX1 have been identified in a human brain (hypothalamus) library and in a human cancer library.Synonyms: Mas-related G-protein coupled receptor member X1, Mrgc, Mrgprc11, Mrgprx1, Sensory neuron-specific G-protein coupled receptor 1, Snsr, Snsr1

Gene ID: 259249

UniProt: Q96LB2