Leukotriene B4 Receptor/BLT antibody

Catalog No. ABIN122284

This Rabbit Polyclonal antibody specifically detects Leukotriene B4 Receptor/BLT in IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Leukotriene B4 Receptor/BLT antibody (ABIN122284)

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leukotriene B4 Receptor/BLT antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Leukotriene B4 Receptor/BLT Antibody

Specificity: Reacts with Leukotriene B4 Receptor BLTR1/P2Y7 (Family: GPCR, Subfamily: Chemoattractant). BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Peptide Immunoaffinity Chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: 5-10 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Leukotriene B4 Receptor/BLT

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Alternative Name: LTB4R / BLTR

Background: BLT1 is a Chemoattractant Receptor that binds the potent chemoattractant leukotriene B4 (LTB4) which is involved in defense against infection. Leukotriene B4 also activates the transcription factor PPAR alpha, resulting in the activation of genes that terminate inflammatory processes. Although BLT1 shows homology to other P2Y receptors, it does not function as a purinoreceptor. Part of the coding regions of BLTR2 (LS57180) have been duplicated and are part of the 5' UTR noncoding regions of the clusters BLT1 (LS190955) and CIDE-B (LS54843) on chromosome 14. BLTR1 has been reported in blood, bone marrow, brain, heart, liver, and skeletal muscle. In addition, BLTR1 has been shown to be highly expressed in leukocytes and lymphoid tissues such as spleen, thymus, and lymph node. ESTs have been isolated from B-cell/lung/testis, breast, colon, eye, kidney, skin, spleen, testis, and tonsil libraries.Synonyms: BLT, BLT1, CMKRL1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, GPR16, LTB4-R 1, Leukotriene B4 receptor 1, P2RY7, P2Y purinoceptor 7, P2Y7

Gene ID: 1241

UniProt: Q15722