DRD4 antibody (C-Term)

Catalog No. ABIN122473

This Rabbit Polyclonal antibody specifically detects DRD4 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for DRD4 antibody (C-Term) (ABIN122473)

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRD4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DRD4 Antibody

Binding Specificity: C-Term

Specificity: Detects Dopamine Receptor D4 (family: GPCR, subfamily: Dopamine)

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: 10 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DRD4

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Alternative Name: Dopamine D4 Receptor

Background: Dopamine Receptor D4 (DRD4), a Dopamine Receptor, has been suggested to affect personality, substance dependence, cognition, and emotion. DRD4 binds the antipsychotic medications clozapine and spiperone. DRD4 knock-out mice are anatomically normal but display altered locomotion and sensitivity to drugs such as clozapine, cocaine, and others. The DRD4 gene contains extensive polymorphisms consisting of variable numbers of tandem repeats (VNTR) in sequences corresponding to the third cytoplasmic loop of the receptor, at least 27 different haplotypes encoding 20 different protein variants have been documented. The frequency of the different variants varies among population groups. Studies have suggested a link between VNTR and human personality traits (such as novelty seeking) and diseases (such as schizophrenia, bipolar affective disorder, and Tourette syndrome), however, considerable debates exist regarding the validity and significance of such associations. DRD4 expression has been reported in various regions of the brain as well as in adrenal gland, artery, eye, heart, kidney, placenta, spinal cord, testis, and vas deferens. ESTs have been isolated from a kidney library.Synonyms: D(2C) dopamine receptor, D(4) dopamine receptor, DRD4

Gene ID: 1815

UniProt: P21917

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity