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CD2 antibody (CD2 Molecule) (PE)

Details for Product anti-CD2 Antibody No. ABIN125677, Supplier: Login to see New
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Synonyms CD2, LFA-2, Ly-37, Ly37, SRBC, T11, CD2R, LFA2, OX-34, OX34
(400), (133), (85), (18), (18), (18), (17), (14), (12), (6), (5), (5), (3), (3), (2), (2), (1)
(421), (112), (78)
Clonality (Clone)
Monoclonal ()
This CD2 antibody is conjugated to PE
(84), (79), (60), (18), (6), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS)
(433), (108), (86), (68), (65), (63), (56), (38), (34), (12), (11), (10), (10), (9), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Quantity 100 tests
Shipping to United States ( )
Availability Will be delivered in 4 to 5 Business Days
Immunogen Human peripheral T cells.
Clone MEM-65
Isotype IgG1
Specificity The antibody MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells, also expressed by all thymocytes.HLDA VI, WS Code T 6T-012
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name CD2 (CD2 Antibody Abstract)
Background CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Product cited in: Espagnolle, Depoil, Zaru et al.: "CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse." in: International immunology, Vol. 19, Issue 3, pp. 239-48, 2007 (PubMed).

Drbal, Hilgert, Cebecauer et al.: "New monoclonal antibodies to human leucocyte surface molecule CD2." in: Folia biologica, Vol. 43, Issue 6, pp. 245-6, 1998 (PubMed).

Background publications Muhammad, Schiller, Forster et al.: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).

Zhu, Dustin, Cairo et al.: "Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion." in: ACS chemical biology, Vol. 1, Issue 10, pp. 649-58, 2006 (PubMed).

Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Catalog No. ABIN125677
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